Topical application of herbal formula for the treatment of ligature-induced periodontitis
김미혜, 최유연, 이혜지, 이해수, 박정철, 양웅모,
소속 상세정보
김미혜 ( Kim Mi-Hye ) - Kyung Hee University College of Korean Medicine Department of Convergence Korean Medical Science
최유연 ( Choi You-Yeon ) - Kyung Hee University College of Korean Medicine Department of Convergence Korean Medical Science
이혜지 ( Lee Hye-Ji ) - Kyung Hee University College of Korean Medicine Department of Convergence Korean Medical Science
이해수 ( Lee Hae-Su ) - Kyung Hee University College of Korean Medicine Department of Convergence Korean Medical Science
박정철 ( Park Jung-Chul ) - Dankook University College of Dentistry Department of Periodontology
양웅모 ( Yang Woong-Mo ) - Kyung Hee University College of Korean Medicine Department of Convergence Korean Medical Science
KMID : 0363020150450040145
Abstract
Purpose: The aim of this study was to investigate the therapeutic effects of a herbal formula, PerioH-035, containing Angelica sinensis, steamed Rehmannia glutinosa, Angelica dahurica, Cimicifuga heracleifolia, and Zanthoxylum piperitum on the periodontal breakdown in a well-established ligature-induced periodontitis model in rats.
Methods: Sprague-Dawley rats were randomly assigned to 1 of 4 groups: NL (non-ligatured), L (ligatured), P1 (ligatured and treated with 1 mg/mL PerioH-035), P100 (ligatured and treated with 100 mg/mL PerioH-035). Periodontitis was induced by placing a ligature around the mandibular first molars. PerioH-035 was topically applied to both sides of the first molar for 2 weeks. The right side of the mandibles was retrieved for micro-computed tomography (CT) and methylene blue staining to analyze alveolar bone loss. The left side of the mandibles was histologically analyzed by TRAP and H&E staining. The MMP-9 mRNA level in gingival tissue was investigated by RT-PCR.
Results: Alveolar bone resorption was significantly reduced in the PerioH-035-treated groups. The number of dense multi-nucleated cells found to be TRAP-positive by staining in the ligatured rats was markedly decreased by PerioH-035 application. In addition, periodontal tissue destruction, especially cementum demineralization, was ameliorated in the P1 and P100 groups. Moreover, gingival tissue from the PerioH-035-treated group showed a decrease in the MMP-9 mRNA level, resulting in recovery of collagen degradation.
Conclusions: These results suggest that PerioH-035 has therapeutic effects on periodontitis, and thus, PerioH-035 shows promise as a treatment for periodontitis.
키워드
Matrix metalloproteinase 9 ; Medicinal herbs ; Osteoclasts ; Periodontitis
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